[PAGID] Herpes virus CMI testing

[Abraham, Roshini S., Ph.D.]

The product that is presently listed on the Cylex website and being
marketed for IVD use is the Immunknow(R) assay that uses the PHA for
stimulation.  They had a product called Cylex T cell memory (R) assay
for research-use only that apparently measured T cell memory response to
a CMV or EBV antigen and there were a couple of abstracts published on
lung and heart-lung transplant patients in 2005 and 2006 by the Univ of
Pittsburgh group, but it is not listed as a product on the company's
website at present.  So, I am not sure whether this product is still
available or not.  

Roshini Abraham
Mayo Clinic
Rochester, MN


-----Original Message-----
From: pagid-bounces at list.clinimmsoc.org
[mailto:pagid-bounces at list.clinimmsoc.org] On Behalf Of Berger, Melvin
Sent: Friday, April 16, 2010 11:51 PM
To: pagid at list.clinimmsoc.org
Subject: RE: [PAGID] Herpes virus CMI testing

I thought they had one with a CMV antigen but the same readout

Melvin Berger, M.D., Ph.D.
Professor of Pediatrics and Pathology
Case Western Reserve University
phone 216 844 3237

Director, Jeffrey Modell Center for Primary Immune Deficiencies
Division of Allergy-Immunology
Rainbow, Babies and Children's Hospital
University Hospitals of Cleveland
RB&C Rm 504, MS 6008B
11100 Euclid Ave.
Cleveland, OH 44106



-----Original Message-----
From: pagid-bounces at list.clinimmsoc.org on behalf of Abraham, Roshini
S., Ph.D.
Sent: Fri 4/16/2010 12:44 PM
To: pagid at list.clinimmsoc.org
Subject: Re: [PAGID] Herpes virus CMI testing
 
The Cylex assay is not CMV-specific....they isolate CD4 T cells from
PBMCs using magnetic beads and they use PHA for stimulation and 24h
later they read ATP production in a luminometer.  It is actually a
rather non-specific assay for several reasons despite the marketing for
immune monitoring of transplant patients.

 

 

Roshini Abraham

Mayo Clinic

Rochester

________________________________

From: pagid-bounces at list.clinimmsoc.org
[mailto:pagid-bounces at list.clinimmsoc.org] On Behalf Of Berger, Melvin
Sent: Friday, April 16, 2010 11:42 AM
To: pagid at list.clinimmsoc.org
Subject: RE: [PAGID] Herpes virus CMI testing

 

I think Cylex might have an assay for lymphocyte activation by CMV they
use for monitoring transplant recipients. 

 

Melvin Berger, M.D., Ph.D.

Adjunct Professor of Pediatrics and Pathology

Case Western Reserve University

phone 216 844 3237

 

Director, Jeffrey Modell Center for Primary Immune Deficiencies

Division of Allergy-Immunology

Rainbow, Babies and Children's Hospital

University Hospitals of Cleveland

RB&C Rm 504, MS 6008B

11100 Euclid Ave.

Cleveland, OH 44106

 

________________________________

From: pagid-bounces at list.clinimmsoc.org on behalf of Saxon, Andy M.D.
Sent: Fri 4/16/2010 10:45 AM
To: 'pagid at list.clinimmsoc.org'
Subject: Re: [PAGID] Herpes virus CMI testing

Thank you Chuck.

 

They have cultured virus from the eye on both CMV eye subjects, one with
the highest load ever reported (there are only a dozen or so cases
reported).

 

On the Zoster subject, you bet we need to confirm it before going
further.  We need to see one of these "episodes' and culture/document
virus in lesions.

 

Andy Saxon, 

UCLA.

 

________________________________

From: pagid-bounces at list.clinimmsoc.org
[mailto:pagid-bounces at list.clinimmsoc.org] On Behalf Of Kirkpatrick,
Charles
Sent: Friday, April 16, 2010 7:34 AM
To: 'pagid at list.clinimmsoc.org'
Subject: Re: [PAGID] Herpes virus CMI testing

Andy, Myron Levin at the Dept. of Pediatrics at Univ. Colorado Health
Sciences Center might be able to help.  He will want confirmation of the
virus.  His e-mail is myron.levin at ucdenver.edu

 

Chuck Kirkpatrick

 

From: pagid-bounces at list.clinimmsoc.org
[mailto:pagid-bounces at list.clinimmsoc.org] On Behalf Of Saxon, Andy M.D.
Sent: Friday, April 16, 2010 2:18 AM
To: PAGID (pagid at list.clinimmsoc.org)
Subject: [PAGID] Herpes virus CMI testing

 

Our ophthalmologists have a couple of other wise normal patients with
isolated CMV anterior chamber of the eye inflammation. While this is
well recognized with Herpes I/II virus, it is very rare with CMV.   The
patients' have antibody to CMV but we'd like to measure cell mediated
immunity to CMV as well.  

 

Does anyone know a lab or group that does measures cell mediated
immunity to CMV?'

 

Similarly, I have an elderly otherwise health lady other than slightly
low % and # CD8 cells [CD8 12%(16-41), 102 (255-1090] and reported
repeated localized Zoster  which is, as usual, not well confirmed in the
records. Clearly we need to establish if it is really Zoster as
generally these cases turn out not to be and her level of antibody to
Zoster. However, in case,

Does anyone know a lab or group that does measures cell mediated
immunity to H. zoster?

 

Andy Saxon, MD 

UCLA School of Medicine

 

 

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