[PAGID] foxp3 ab

Wed May 10 13:25:45 EDT 2006

Sergio,
The eBioscience antibody has worked in Cincinnati as well.
Both the FITC and PE conjugates work, clone PCH101.
Don't use the hFOXY clone- it is non-specific.
Kim

Kimberly Risma MD, PhD
Assistant Professor
Allergy Immunology
Childrens Hospital Medical Center
3333 Burnet Ave, mlc 2000
Cincinnati, OH 45229
Kimberly.Risma at cchmc.org

>>> TFleishe at cc.nih.gov 05/10/06 8:19 AM >>>

Sergio,We have been using the eBioscience product for the past 8 months
and feel that it works well.  You could email Bosco in Sao Paulo (use
his old NIH email address) for his input as he has done most of the
work.Tom Thomas A. Fleisher, M.D.Chief, Department of Laboratory
MedicineClinical Center, NIHBethesda, MD 20892-1508Tel 301 496-5668Fax
301 402-1612

From: Sergio Rosenzweig [mailto:sdrosenzweig at yahoo.com] 
Sent: Tuesday, May 09, 2006 6:48 PM
To: pagid at clinimmsoc.org
Subject: [PAGID] foxp3 ab
 Dear colleagues,
Does anybody can recommend me a reliable and commercially available 
anti human foxp3 ab for flow?
Thanks in advance,
Sergio

Sergio D. Rosenzweig, MD
Servicio de Inmunologia
Hoapital Nacional de Pediatria "J. P. Garrahan"
Buenos Aires, Argentina
srosenzweig at garrahan.gov.ar

"Haines, Kathleen, M.D." <KHaines at humed.com> wrote:
I had the same thought...and I've seen SOJA with low platelets. 

Kathleen A. Haines, MD
Section Chief, Pediatric Immunology
Pediatric Rheumatology and Immunology
Hackensack University Medical Center
30 Prospect Ave.
Hackensack, NJ 07601

Tel: 201-996-5306
Fax: 201-996-9815

-----Original Message-----
From: pagid-bounces at clinimmsoc.org
[mailto:pagid-bounces at clinimmsoc.org] On
Behalf Of Christine Seroogy
Sent: Wednesday, March 29, 2006 10:13 AM
To: pagid at clinimmsoc.org
Subject: Re: [PAGID] (no subject)

Have you considered systemic JRA in this patient? Chris

On Mar 29, 2006, at 8:37 AM, Sullivan, John (Office of Research)
wrote:

> Colleagues; I need your help with a difficult case; Patient 
> presented one year ago as a 2yo female from India; admitted with 
> fever, failure to thrive[ht and wt <3%], diffuse lymphadenopathy, 
> splenomegaly and arthralgias and history of intermittent rashes; 
> laboratory studies: WBC 17.4, Hgb 7.5, HCT 23, MCV 58, MCH 19.2, 
> MCHC33, Platelets 54K, Differential, Neutophils 67%,Lymphocytes 
> 28%,Monocytes 4%,Eosinophils 1%; ESR 48; IgG1780, normal IgA, IgM, 
> IgD, IgE; Total Hemolytic Complement normal; AST 74, ALT, 21, LDH 
> 1192, Tot Protein 6.2, Albumin 2.1, normal renal function; elevated 
> FDP and D- dimer, normal AT, compatible with active fibrinolysis 
> and early DIC; HIV serology negative; Bone Marrow, cellular marrow, 
> mild dyserythropoiesis, no evidence of malignancy, normal 
> karyotype, normal T and B cells; Lymph Node Biopsy, reactive lymph 
> node with changes consistent with chronic stimulation and 
> involution; Peripheral Blood, CD T Cells 78%, CD 4 T Cells 35%, CD 
> 8 TCells 45%, CD 4/CD 8 0.78.
>
> Diagnoses: Immune Activation Syndrome secondary to ? viral 
> infection; Alpha Thalassemia Trait
>
> Treatment: Begun in March 05 on Prednisone 2mg/kg/day with slow but 
> progressive improvement till al symptoms resolved except continued 
> HT and Wt <3%; tapered to 1mg/kg/day in August 05; then to QOD 
> prednisone; we were tapering off QOD regimen when over past two 
> weeks at 9mg QOD patient again started fevers, elevated ESR and 
> CRP, LFTs, albumin 2.8, Total protein 6.9; currently back on 
> Prednisone 1mg/kg/day. Parents frustrated as are Dr and Nurse; not 
> sure what we are treating at this point; any help will be 
> appreciated; thanks, John.
>
>
>
>
>
>
> John L Sullivan MD
> Professor of Pediatrics
> University of Massachusetts Medical School
> 55 Lake Avenue North
> Worcester MA 01655
> Phone 508-856-1572
> Fax 508-856-5004
> email john.sullivan at umassmed.edu
>
>
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