[PAGID] Complete DiGeorge options

[David Nelson]

I would suppress the GVHD with saturating doses of humanized anti-Tac 
and then let host cells develop independently of IL-2 as they do in 
the IL-2Ra KO mouse or IL-2Ra deficient humans.

Then I would taper off the anti-Tac and let the host IL-2Ra+ Tregs 
develop to prevent the autoimmunity.  The engrafted cells should be 
very susceptible to IL-2 deprivation apoptosis.  Saturation could be 
examined using a combination of directly-conjugated anti-Tac and 
7G7/B6 staining of IL-2Ra.  When saturated with anti-Tac there is no 
anti-Tac staining but 7G7/B6 still reveals the IL-2Ra positive cells.

This is what we have don for pediatric ATL patients in "autocrine phase".

David L. Nelson, M. D.
Metabolism Branch, CCR, NCI
NIH
Bethesda, MD 20892