[CIS-PAGID] Neutrophil Migration Assay. .

[Kate Sullivan]

We use it as a screen for SDS and HIGE.  Yes- it is an assay that can be confounded by many things but it is light years cheaper than genetic testing and as a screen it is not bad.

Kate
Kate Sullivan, MD PhD
Professor of Pediatrics
ARC 1216 Immunology CHOP
3615 Civic Center Blvd.
Philadelphia, PA 19104
215-590-1697


On Dec 22, 2010, at 4:24 PM, Conley, Mary Ellen wrote:


> Thanks to Jack and Ron,

> But wouldn't you agree the chemotaxis was not the sole or most reliable abnormality? In both setting, this might be considered a research study.

> Mary Ellen

> 

> 

> 

> 

> 

> 

> Mary Ellen Conley, MD

> Department of Immunology/ Mail Stop 351

> St. Jude Children's Research Hospital

> 262 Danny Thomas Place

> Memphis, TN 38105-3678

> FAX  901-595-3977

> TEL  901-595-2576

> 

> 

> -----Original Message-----

> From: pagid-bounces at list.clinimmsoc.org [mailto:pagid-bounces at list.clinimmsoc.org] On Behalf Of Harbeck, Ron

> Sent: Wednesday, December 22, 2010 2:47 PM

> To: pagid at list.clinimmsoc.org

> Subject: Re: [CIS-PAGID] Neutrophil Migration Assay. .

> 

> 

> We helped in the identification of two patients with a Rac2 mutations with impaired chemotaxis and reported each:

> 

> Accetta D, Syverson G, Bonacci B, Reddy S, Bengtson C, Surfus J, Harbeck R, Huttenlocher A, Grossman W, Routes J, Verbsky J. Human phagocyte defect caused by a Rac2 mutation detected by means of neonatal screening for T-cell lymphopenia.  J Allergy Clin Immunol. 2010 Dec 15. [Epub ahead of print]. 

> 

> 

> Ambruso DR, Knall C, Abell AN, Panepinto J, Kurkchubasche A, Thurman G, Gonzalez-Aller C, Hiester A, deBoer M, Harbeck RJ, Oyer R, Johnson GL, Roos D. Human neutrophil immunodeficiency syndrome is associated with an inhibitory Rac2 mutation. Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4654-9.

> 

> Ron Harbeck

> National Jewish Health

> 

> -----Original Message-----

> From: pagid-bounces at list.clinimmsoc.org

> [mailto:pagid-bounces at list.clinimmsoc.org] On Behalf Of Conley, Mary Ellen

> Sent: Wednesday, December 22, 2010 1:40 PM

> To: 'pagid at list.clinimmsoc.org'

> Subject: Re: [CIS-PAGID] Neutrophil Migration Assay. .

> 

> Like Charlotte, I was wondering why you wanted to look at chemotaxis.

> What do people think are the indications for this assay and are there any treatable disorders in which chemotaxis is the sole or most reliable abnormality?

> Mary Ellen

> 

> 

> 

> 

> Mary Ellen Conley, MD

> Department of Immunology/ Mail Stop 351

> St. Jude Children's Research Hospital

> 262 Danny Thomas Place

> Memphis, TN 38105-3678

> FAX  901-595-3977

> TEL  901-595-2576

> 

> 

> -----Original Message-----

> From: pagid-bounces at list.clinimmsoc.org

> [mailto:pagid-bounces at list.clinimmsoc.org] On Behalf Of Cunningham-Rundles, Charlotte

> Sent: Wednesday, December 22, 2010 2:34 PM

> To: PAGID

> Subject: Re: [CIS-PAGID] Neutrophil Migration Assay. .

> 

> I think that CHOP does but I can't think of a use for it

> 

> Charlotte Cunningham-Rundles MD PhD

> Departments of Medicine, Pediatrics

> The Immunology Institute

> Mount Sinai School of Medicine

> 1425 Madison Avenue,

> New York City, New York, 10029

> 212 659 9268 (phone)

> 212 987 5593 (fax)

> 

> 

>> From: <raas0027 at umn.edu>

>> Reply-To: PAGID <pagid at list.clinimmsoc.org>

>> Date: 22 Dec 2010 13:46:09 -0600

>> To: PAGID <pagid at list.clinimmsoc.org>

>> Subject: [CIS-PAGID] Neutrophil Migration Assay

>> 

>> What commercial or academic labs in the US will perform neutrophil 

>> migration/chemotaxis studies?

>> 

>> 

>> --

>> Jason Raasch, MD

>> 

>> Midwest Immunology Clinic

>> 15700 37th Ave N

>> Suite 110

>> Plymouth, MN 55446

>> 

>> (Phone) 763.577.0008

>> (FAX)   763.5770192

> 

> 

> 

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