[CIS-PAGID] Neutrophil Migration Assay. .

[Jack Bleesing]

True. I mentioned it from the perspective that certain assays that seem to belong to the Immunological Laboratory may have other (sometimes it seems "better") applications outside our sphere of influence.
 
Never been very good at placing NK-cell function tests (and result) amongst the dots of an immunodeficiency workup, but in HLH conditions, it sure has proven its value.
 
J
 
---------------------------------------------------------------------------
Jack J.H. Bleesing, M.D., Ph.D.
Associate Professor of Pediatrics
Cincinnati Children's Hospital Medical Center
Division of Bone Marrow Transplantation and Immune Deficiency
3333 Burnet Avenue, MLC 7015
Cincinnati, OH 45229
513-636-4266 (phone)
513-636-3549 (fax)



>>> "Conley, Mary Ellen" <maryellen.conley at STJUDE.ORG> 12/22/2010 4:24 PM >>>

Thanks to Jack and Ron,
But wouldn't you agree the chemotaxis was not the sole or most reliable abnormality? In both setting, this might be considered a research study.
Mary Ellen






Mary Ellen Conley, MD
Department of Immunology/ Mail Stop 351
St. Jude Children's Research Hospital
262 Danny Thomas Place
Memphis, TN 38105-3678
FAX  901-595-3977
TEL  901-595-2576


-----Original Message-----
From: pagid-bounces at list.clinimmsoc.org [mailto:pagid-bounces at list.clinimmsoc.org] On Behalf Of Harbeck, Ron
Sent: Wednesday, December 22, 2010 2:47 PM
To: pagid at list.clinimmsoc.org 
Subject: Re: [CIS-PAGID] Neutrophil Migration Assay. .


We helped in the identification of two patients with a Rac2 mutations with impaired chemotaxis and reported each:

Accetta D, Syverson G, Bonacci B, Reddy S, Bengtson C, Surfus J, Harbeck R, Huttenlocher A, Grossman W, Routes J, Verbsky J. Human phagocyte defect caused by a Rac2 mutation detected by means of neonatal screening for T-cell lymphopenia.  J Allergy Clin Immunol. 2010 Dec 15. [Epub ahead of print]. 


Ambruso DR, Knall C, Abell AN, Panepinto J, Kurkchubasche A, Thurman G, Gonzalez-Aller C, Hiester A, deBoer M, Harbeck RJ, Oyer R, Johnson GL, Roos D. Human neutrophil immunodeficiency syndrome is associated with an inhibitory Rac2 mutation. Proc Natl Acad Sci U S A. 2000 Apr 25;97(9):4654-9.

Ron Harbeck
National Jewish Health

-----Original Message-----
From: pagid-bounces at list.clinimmsoc.org 
[mailto:pagid-bounces at list.clinimmsoc.org] On Behalf Of Conley, Mary Ellen
Sent: Wednesday, December 22, 2010 1:40 PM
To: 'pagid at list.clinimmsoc.org'
Subject: Re: [CIS-PAGID] Neutrophil Migration Assay. .

Like Charlotte, I was wondering why you wanted to look at chemotaxis.
What do people think are the indications for this assay and are there any treatable disorders in which chemotaxis is the sole or most reliable abnormality?
Mary Ellen




Mary Ellen Conley, MD
Department of Immunology/ Mail Stop 351
St. Jude Children's Research Hospital
262 Danny Thomas Place
Memphis, TN 38105-3678
FAX  901-595-3977
TEL  901-595-2576


-----Original Message-----
From: pagid-bounces at list.clinimmsoc.org 
[mailto:pagid-bounces at list.clinimmsoc.org] On Behalf Of Cunningham-Rundles, Charlotte
Sent: Wednesday, December 22, 2010 2:34 PM
To: PAGID
Subject: Re: [CIS-PAGID] Neutrophil Migration Assay. .

I think that CHOP does but I can't think of a use for it

Charlotte Cunningham-Rundles MD PhD
Departments of Medicine, Pediatrics
The Immunology Institute
Mount Sinai School of Medicine
1425 Madison Avenue,
New York City, New York, 10029
212 659 9268 (phone)
212 987 5593 (fax)



> From: <raas0027 at umn.edu>

> Reply-To: PAGID <pagid at list.clinimmsoc.org>

> Date: 22 Dec 2010 13:46:09 -0600

> To: PAGID <pagid at list.clinimmsoc.org>

> Subject: [CIS-PAGID] Neutrophil Migration Assay

>

> What commercial or academic labs in the US will perform neutrophil 

> migration/chemotaxis studies?

>

>

> --

> Jason Raasch, MD

>

> Midwest Immunology Clinic

> 15700 37th Ave N

> Suite 110

> Plymouth, MN 55446

>

> (Phone) 763.577.0008

> (FAX)   763.5770192




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