[PAGID] Cylex test

[Abraham, Roshini S., Ph.D.]

We do the Cylex assay here in our lab but at present it is being used
only for research purposes and we are in the process of doing some
prospective studies in SOT/BMT to develop guidelines for clinical use. I
have used it in a DiGeorge patient to assess lymphocyte
proliferation/function.  One cannot totally rely on the company-set
cutoffs, so it is helpful to have prospective data to effectively guide
clinical use but it has the potential to be helpful in such settings.

 

Roshini

 

 

Roshini Sarah Abraham, Ph.D., D(ABMLI)

Cellular and Molecular Immunology Laboratory

Department of Laboratory Medicine and Pathology

Mayo Clinic

Hilton 210 e

Ph (office): 507-266-9292

Ph (Secy): 507-284-4055

Fax:507-266-4088

________________________________

From: pagid-bounces at list.clinimmsoc.org
[mailto:pagid-bounces at list.clinimmsoc.org] On Behalf Of MacGinnitie,
Andrew
Sent: Wednesday, July 02, 2008 8:27 AM
To: pagid at list.clinimmsoc.org
Subject: [PAGID] Cylex test

 

I wonder if anyone is using or has thoughts on this test

 

http://www.cylex.net/assayprinciple.html

 

Basically it is a simplified, automated mitogen stimulation of CD4
cells.  It apparently is fast (24 hours or less) and requires much less
blood than traditional proliferation assays.  At our institution it is
also done in-house whereas we have to send out traditional proliferation
assays

 

Our bone marrow transplantation colleagues are using it routinely
because it is so convenient and it is apparently also being marketed to
guide immunosupression after solid organ transplant.  

 

I often struggle about whether I need to send proliferation on a kid who
I don't have a super-high suspicion (e.g. DiGeorge with low, but present
T-cells) and wonder if this might not be a better alternative


Andy

 

Andrew J. MacGinnitie MD PhD

Assistant Professor of Pediatrics

Division of Pulmonary Medicine, Allergy and Immunology

Children's Hospital of Pittsburgh

3705 Fifth Ave, Pittsburgh, PA 15213

andrew.macginnitie at chp.edu

412/692-8903 (office)

412/692-8499 (fax)

 

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