[CIS-PAGID] Neutrophil Migration Assay. .

[Church, Joseph]

There are rare primary disorders of leukotaxis that will not be
identified with adhesion molecule analysis.  

In 1991 we identified a patient with a neutrophil motility defect
secondary to abnormal actin polymerization (Blood 1991;78:1338-46).  The
only abnormal functional assay was a profound neutrophil chemotactic
defect.  The patient underwent successful bone marrow transplantation.

Joe Church
Children's Hospital Los Angeles

-----Original Message-----
From: pagid-bounces at list.clinimmsoc.org
[mailto:pagid-bounces at list.clinimmsoc.org] On Behalf Of Harbeck, Ron
Sent: Wednesday, December 22, 2010 12:47 PM
To: pagid at list.clinimmsoc.org
Subject: Re: [CIS-PAGID] Neutrophil Migration Assay. .


We helped in the identification of two patients with a Rac2 mutations
with impaired chemotaxis and reported each:

Accetta D, Syverson G, Bonacci B, Reddy S, Bengtson C, Surfus J, Harbeck
R, Huttenlocher A, Grossman W, Routes J, Verbsky J. Human phagocyte
defect caused by a Rac2 mutation detected by means of neonatal screening
for T-cell lymphopenia.  J Allergy Clin Immunol. 2010 Dec 15. [Epub
ahead of print]. 


Ambruso DR, Knall C, Abell AN, Panepinto J, Kurkchubasche A, Thurman G,
Gonzalez-Aller C, Hiester A, deBoer M, Harbeck RJ, Oyer R, Johnson GL,
Roos D. Human neutrophil immunodeficiency syndrome is associated with an
inhibitory Rac2 mutation. Proc Natl Acad Sci U S A. 2000 Apr
25;97(9):4654-9.

Ron Harbeck
National Jewish Health

-----Original Message-----
From: pagid-bounces at list.clinimmsoc.org
[mailto:pagid-bounces at list.clinimmsoc.org] On Behalf Of Conley, Mary
Ellen
Sent: Wednesday, December 22, 2010 1:40 PM
To: 'pagid at list.clinimmsoc.org'
Subject: Re: [CIS-PAGID] Neutrophil Migration Assay. .

Like Charlotte, I was wondering why you wanted to look at chemotaxis.
What do people think are the indications for this assay and are there
any treatable disorders in which chemotaxis is the sole or most reliable
abnormality?
Mary Ellen




Mary Ellen Conley, MD
Department of Immunology/ Mail Stop 351
St. Jude Children's Research Hospital
262 Danny Thomas Place
Memphis, TN 38105-3678
FAX  901-595-3977
TEL  901-595-2576


-----Original Message-----
From: pagid-bounces at list.clinimmsoc.org
[mailto:pagid-bounces at list.clinimmsoc.org] On Behalf Of
Cunningham-Rundles, Charlotte
Sent: Wednesday, December 22, 2010 2:34 PM
To: PAGID
Subject: Re: [CIS-PAGID] Neutrophil Migration Assay. .

I think that CHOP does but I can't think of a use for it

Charlotte Cunningham-Rundles MD PhD
Departments of Medicine, Pediatrics
The Immunology Institute
Mount Sinai School of Medicine
1425 Madison Avenue,
New York City, New York, 10029
212 659 9268 (phone)
212 987 5593 (fax)



> From: <raas0027 at umn.edu>

> Reply-To: PAGID <pagid at list.clinimmsoc.org>

> Date: 22 Dec 2010 13:46:09 -0600

> To: PAGID <pagid at list.clinimmsoc.org>

> Subject: [CIS-PAGID] Neutrophil Migration Assay

>

> What commercial or academic labs in the US will perform neutrophil

> migration/chemotaxis studies?

>

>

> --

> Jason Raasch, MD

>

> Midwest Immunology Clinic

> 15700 37th Ave N

> Suite 110

> Plymouth, MN 55446

>

> (Phone) 763.577.0008

> (FAX)   763.5770192




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